5-Amino-1MQ Research Compound (NNMT Inhibitor)
Powder Vial Format • ≥99% HPLC Purity • Ships in 1–2 Business Days • U.S. Warehouses
5-Amino-1MQ (5-Amino-1-methylquinolinium iodide, CAS 42464-96-0) is a synthetic small-molecule quaternary quinolinium compound supplied by Nextday Peptides in powder vial format for laboratory and analytical research applications. As a selective substrate site-targeting inhibitor of nicotinamide N-methyltransferase (NNMT) — the cytosolic enzyme responsible for the methylation of nicotinamide using S-adenosylmethionine (SAM) as a methyl donor — 5-Amino-1MQ is studied as a tool compound for investigating NNMT enzyme activity, NAD⁺ salvage pathway interactions, and methylation pathway dynamics in controlled in-vitro experimental systems. Each vial is independently verified to ≥99% HPLC purity with a Certificate of Analysis available on request. For scientific research use only.
Compound Specifications
| Compound Name | 5-Amino-1MQ / 5-Amino-1-methylquinolinium iodide |
| Chemical Name | 5-Amino-1-methylquinolinium iodide |
| Target Enzyme | NNMT — Nicotinamide N-methyltransferase (cytosolic) |
| Inhibitor Type | Selective substrate site-targeting NNMT inhibitor |
| IC50 (NNMT) | 1.2 μM (Sigma-Aldrich reference; 50 μM SAM, 100 μM NCA assay conditions) |
| Molecular Formula | C₁₀H₁₁IN₂ (iodide salt) |
| Molecular Weight | 286.11 g/mol (iodide salt) |
| CAS Number | 42464-96-0 |
| Form | Powder vial |
| Appearance | Yellow to off-white crystalline powder |
| Purity | ≥99% (HPLC verified) |
| Solubility | DMSO (~2 mg/mL); limited aqueous solubility (ionic quaternary ammonium compound) |
| Storage | 2–8°C; protect from light and moisture |
| Synonyms | 5-amino-1MQ, 5A-1MQ, NNMTi, NNMT inhibitor 1MQ |
Why Research Laboratories Select 5-Amino-1MQ
5-Amino-1MQ is the most widely referenced selective NNMT inhibitor in current enzyme biology and methylation pathway research. Its substrate site-targeting mechanism — inhibiting NNMT at the nicotinamide binding site — provides selectivity over related methyltransferases and other enzymes in the NAD⁺ salvage pathway, making it a precise tool compound for studying NNMT-specific enzymatic activity in controlled laboratory systems.
- Selective NNMT inhibitor — substrate site-targeting mechanism with IC50 of 1.2 μM in reference assay conditions
- Selectivity over related methyltransferases — does not significantly affect other enzymes in the NAD⁺ salvage pathway at research concentrations
- Reduces intracellular 1-methylnicotinamide (1-MNA) formation in cell-based assay models
- Studied in adipocyte, liver, and skeletal muscle cell-based research models examining NNMT-dependent methylation pathway dynamics
- Referenced at Sigma-Aldrich (SML2832) and in peer-reviewed literature on NNMT enzyme biology
- Membrane-permeable quaternary quinolinium scaffold — studied for intracellular NNMT target engagement in cell-based models
- Batch and lot identifiers on all labeling for full laboratory documentation compliance
Research Background
5-Amino-1MQ was first characterized as a selective NNMT inhibitor in published research literature in 2018. Rhoads et al. (2018) characterized the substrate site-targeting mechanism and selectivity profile of the 5-amino-1-methylquinolinium series in Cell Chemical Biology. Subsequent peer-reviewed preclinical studies have examined its use as a tool compound for investigating NNMT enzyme activity in adipose, liver, and skeletal muscle cell-based research models under controlled laboratory conditions. All findings described here are from controlled preclinical research settings. All information provided here is for educational and reference purposes only and does not constitute medical or clinical guidance. This compound is not approved by the FDA for any use and is supplied strictly for in-vitro laboratory research use only.
Reference sources: Sigma-Aldrich — 5-Amino-1MQ (SML2832) | NIH PMC — NNMT Inhibitor Research
⚠️ Regulatory Notice
5-Amino-1MQ is an unapproved new drug compound. It has not been evaluated by the FDA for safety or efficacy in humans. This product is supplied strictly for controlled in-vitro laboratory research use only by qualified research professionals in certified laboratory facilities. It is not for human or veterinary use under any circumstances. All applicable federal, state, and institutional regulations governing the handling and use of unapproved research compounds must be followed.
Shipping & Fulfillment
- Ships from U.S. warehouses — Florida, North Carolina, and California
- Overnight and 2-day shipping available at checkout
- Same-day processing on orders placed before 3:30 PM ET
- Discreet packaging with full tracking provided
- Bulk research orders welcome — contact us for volume pricing
Storage & Handling
- Store at 2–8°C away from light and moisture
- Handle only in a laboratory setting with appropriate PPE — nitrile gloves and safety goggles required
- Iodide salt form is a chemical irritant — H302 (harmful if swallowed), H315 (causes skin irritation), H319 (causes serious eye irritation)
- Dissolve in DMSO (~2 mg/mL) — limited aqueous solubility due to ionic quaternary ammonium structure
- Keep containers tightly sealed when not in use
Frequently Asked Questions
What is 5-Amino-1MQ?
5-Amino-1MQ (5-Amino-1-methylquinolinium iodide, CAS 42464-96-0) is a synthetic small-molecule quaternary quinolinium compound that functions as a selective substrate site-targeting inhibitor of nicotinamide N-methyltransferase (NNMT). It is supplied strictly for controlled in-vitro scientific and analytical laboratory research use only by qualified research professionals.
What is NNMT and why is it studied in laboratory research?
Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme that catalyzes the methylation of nicotinamide using S-adenosylmethionine (SAM) as a methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH). Research examining NNMT activity studies its role in nicotinamide metabolism, SAM availability for cellular methylation reactions, and NAD⁺ salvage pathway interactions in controlled laboratory systems.
How does 5-Amino-1MQ inhibit NNMT?
5-Amino-1MQ targets the substrate binding site of NNMT — the nicotinamide binding pocket — competitively inhibiting nicotinamide methylation with an IC50 of 1.2 μM under reference assay conditions. This substrate site-targeting mechanism provides selectivity over related methyltransferases and other NAD⁺ salvage pathway enzymes in laboratory assay systems.
Is 5-Amino-1MQ FDA approved?
No. 5-Amino-1MQ is an unapproved new drug compound that has not been evaluated by the FDA for safety or efficacy in humans. It is supplied strictly for in-vitro laboratory research use only. It is not for human or veterinary use under any circumstances.
What purity is your 5-Amino-1MQ?
Every batch is independently verified to ≥99% HPLC purity. A Certificate of Analysis is available for each lot.
How fast does Nextday Peptides ship?
We ship from U.S. warehouses in Florida, North Carolina, and California. Overnight and 2-day shipping options are available. Orders placed before 3:30 PM ET are processed same day.
Are bulk orders available?
Yes. We accommodate bulk research orders. Contact us directly for volume pricing and availability.
Is this product approved for medical use?
No. This product is an unapproved research compound for in-vitro laboratory use only. It is not approved for human or veterinary use and is not intended for diagnostic, therapeutic, or clinical applications of any kind.
What other compounds does Nextday Peptides carry?
We stock a full range of research compounds including NAD+, SLU-PP-332, MOTS-C, and Glutathione GSH. View our full research compounds catalog.
For Research Use Only — Not for human or veterinary consumption • Not evaluated by the FDA • Unapproved new drug compound — supplied by Nextday Peptides for controlled in-vitro laboratory research use only by qualified research professionals
