Research Use Only — Not for Human Consumption. This article focuses exclusively on laboratory characterization, handling, and quality control for 5-Amino-1MQ. It does not address clinical, therapeutic, or veterinary applications. Use this information to support safe and reproducible benchwork in research environments concerned with small-molecule evaluation.
Overview
5-Amino-1MQ is a small, heteroaromatic compound encountered in chemical and biochemical research. Investigators handle it as a research reagent and a target for analytical study rather than as an approved therapeutic. Throughout laboratory workflows researchers treat 5-Amino-1MQ under a Research Use Only — Not for Human Consumption classification and apply standard small-molecule QC and handling practices.
Chemical Structure
The molecule commonly referred to as 5-Amino-1MQ consists of a substituted aromatic heterocycle with an amino group at the 5-position and a methyl modification at the 1-position on the parent scaffold. These features confer aromaticity, localized basicity near the amino substituent, and potential for salt formation or protonation depending on pH. Analytical identity is most reliably confirmed by a combination of spectroscopic methods (1H/13C NMR, LC‑MS) and chromatographic purity assessment (HPLC or UPLC).
In research workflows, labs document tautomeric possibilities, salt vs. free-base forms, and any stereochemical concerns; this informs solubility choices, stability testing, and method development for assays. Always consult the supplier COA and independent spectral data when confirming structural identity for 5-Amino-1MQ.
Solubility & Stability
Solubility profiling for 5-Amino-1MQ should begin with a solvent screen that progresses from polar aprotic solvents to aqueous systems. Typical practice is to prepare concentrated DMSO stocks (for example, 10–50 mM) because DMSO dissolves many heteroaromatic small molecules efficiently. Ethanol or methanol can be alternative organic solvents. Aqueous buffer solubility is often limited; pH adjustments, co-solvents, or formulation as a salt may be required for higher aqueous concentrations.
Stability depends on factors such as light exposure, oxygen, moisture, temperature, and pH. Labs routinely evaluate stability by: accelerated forced-degradation studies (heat, light, oxidants), short-term bench-top stability at ambient conditions, and long-term frozen storage stability. For practical handling, prepare single-use aliquots in inert vials, avoid repeated freeze–thaw cycles, and track time-in-solution for experimental records. Again: Research Use Only — Not for Human Consumption.
Lab Storage Guidelines
Best-practice storage for 5-Amino-1MQ follows small-molecule conventions. Store dry, in airtight containers with desiccant, and protect from light if the compound shows photolability. For long-term preservation, hold samples at −20 °C or −80 °C under inert gas or tightly sealed conditions to limit air and moisture exposure. Short-term working stocks may be kept at 4 °C for hours to days depending on stability data.
Label containers with compound name, lot number, concentration, solvent, date aliquoted, and principal investigator. Maintain a sample inventory and log any freeze–thaw events. Use appropriate PPE—gloves, eye protection, and lab coat—and handle powders and concentrated stocks in a fume hood to reduce inhalation risk. Always consult the material safety data sheet (MSDS) provided by the supplier before beginning work with 5-Amino-1MQ.
COA Review
Certificates of Analysis (COAs) are primary documents for assessing batch quality of 5-Amino-1MQ. A complete COA will typically include:
- Compound identity: LC‑MS trace and observed m/z, 1H/13C NMR spectra, and retention time comparisons.
- Purity: % area by HPLC/UPLC with method parameters (column, solvent, gradient) and chromatograms.
- Water content: Karl Fischer titration where relevant.
- Residual solvents: GC analysis against ICH limits.
- Elemental impurities or heavy metals: ICP-MS if required for the application.
- Batch information: lot number, manufacture date, recommended storage.
When reviewing a COA for 5-Amino-1MQ, compare supplied analytical traces to in-house reference data when possible. If the COA shows atypical peaks, inconsistent mass data, or unexplained impurities, request supplementary raw data or perform orthogonal testing (e.g., LC‑MS and NMR) before proceeding. Remember: Research Use Only — Not for Human Consumption.
Batch Testing and How Labs Evaluate Small Molecules
Laboratories evaluate batches of small molecules like 5-Amino-1MQ using a tiered testing strategy: identity confirmation, purity quantification, and stability profiling. Typical assays include high-resolution LC‑MS for molecular weight and purity, HPLC with UV or PDA detection for percent area, and NMR for structural confirmation. Forced‑degradation studies (acid/base, oxidative, thermal, photolytic) help map degradation pathways and establish analytical specificity.
Acceptance criteria are set by the laboratory’s quality policy and project needs; common thresholds require ≥95% purity by HPLC unless experimental design tolerates lower purity and impurities are characterized. Track batch-specific data in an internal database to support reproducibility across experiments involving 5-Amino-1MQ.
Handling Techniques and Reconstitution Considerations
When preparing working solutions of 5-Amino-1MQ, weigh powders quickly to minimize atmospheric exposure and use dry, low-adsorption vials. Dissolve the compound in the chosen solvent with gentle vortexing, sonication, or brief warming in a controlled water bath if needed. Avoid prolonged heating; many small molecules degrade under excess heat.
Filtered sterile solutions can be produced by passing solvent stocks through a 0.22 µm filter, although note that some compounds adsorb to filter membranes—validate recovery after filtration. For long-term use, aliquot into single-use volumes, snap-freeze in liquid nitrogen if appropriate, and store under conditions validated by stability testing. Maintain detailed documentation of solvent type, final concentration, pH, and date prepared for any 5-Amino-1MQ stock solution.
Research FAQs
Research Use Only — Not for Human Consumption.
- Q: What solvents are recommended for preparing concentrated stocks of 5-Amino-1MQ?
- A: Begin with polar aprotic solvents such as DMSO for concentrated stocks. Ethanol or methanol may be alternatives. Verify solubility by small-scale testing and document any need for co-solvents or pH adjustment for aqueous formulations.
- Q: How should I confirm identity and purity upon receipt?
- A: Cross-check the supplier COA with in-house methods—LC‑MS for molecular weight and mass purity, HPLC for percent area, and NMR for structural confirmation. If discrepancies appear, perform orthogonal testing before use.
- Q: What storage temperature is recommended?
- A: Store sealed, desiccated samples at −20 °C or −80 °C for long-term stability; short-term working stocks can be held at 4 °C if validated. Protect from light and moisture.
- Q: Are there special handling precautions?
- A: Use standard chemical safety practices: lab coat, gloves, safety glasses, and a fume hood when weighing or preparing concentrated stocks. Review the MSDS and keep accurate inventory and traceability records.
- Q: Where does the phrase “Research Use Only — Not for Human Consumption” apply?
- A: That designation should appear on all labels, purchase orders, and experimental records for 5-Amino-1MQ to make clear that the material is intended solely for laboratory research and not for any use in humans or animals.
Research Use Only — Not for Human Consumption. Careful QC, documented handling, and adherence to COA data are the backbone of reproducible work with 5-Amino-1MQ. Use validated analytical methods, track lot-specific performance, and store the compound under conditions supported by stability testing to preserve sample integrity for research applications.